Induction of resistance to rice leaf blast by avirulent isolates of Magnaporthe oryzae

Autores

  • Marta Cristina Corsi Filippi Embrapa Arroz e Feijão
  • Gisele Barata Silva Universidade Federal Rural da Amazônia
  • Hérica Fernandes Viana Universidade Federal de Goiás
  • Valácia Lemes Silva-Lobo Embrapa Arroz e Feijão
  • Márcio Vinicius Carvalho Barros Côrtes Embrapa Arroz e Feijão
  • Anne Sitarama Prabhu Embrapa Arroz E Feijão

Resumo

Rice blast (Magnaporthe oryzae) disease can cause losses of up to 100% in
grain production. A sustainable management should consider alternative control measures.
In the present work, biotic resistance induction to blast disease was studied by comparing
disease severity and quantifying some pathogenesis-related proteins (PRPs). Rice plants of
cultivars Metica-1 and Cica-8 were sprayed with biotic resistance inducers (incompatible
isolates of M. oryzae) 48 h before inoculation with challenging isolates (compatible isolates
of M. oryzae) under greenhouse conditions. For both cultivars, Metica-1 and Cica-8, the
area under the disease progress curve was reduced by more than 80% when plants were
induced with avirulent isolates of Magnaporthe oryzae. The biotic inducers also promoted
an increase in the activity of peroxidase (POX), β-1.3-glucanase (GLU), chitinase (CHI), and
phenylalanine ammonia-lyase (PAL). Systemic expression of resistance was also observed.

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Biografia do Autor

Marta Cristina Corsi Filippi, Embrapa Arroz e Feijão

Fitopatologia

Gisele Barata Silva, Universidade Federal Rural da Amazônia

ÌCA/Laboratorio de Proteção de Plantas

Valácia Lemes Silva-Lobo, Embrapa Arroz e Feijão

Fitopatologia

Anne Sitarama Prabhu, Embrapa Arroz E Feijão

Fitopatologia

Referências

DAUGROIS, J.H.; LAFITTE, C.; BARTHE, J.P.; TOUZE, A. Induction of -1,4-glucanase and chitinase activity in compatible and incompatible interactions between Colletotrichum lindemuthianum and beans cultivars. Journal of Phytopathology, v.130, p.225-234, 1990.

DICKERSON, D.P.; PASCHOLATI S.F.; HAGERMAN, A.E.; BUTLER, L.G.; NICHOLSON, R.L. Phenylalanine ammonia-lyase and hydroxy cinnamate CoA ligase in maize mesocotyls inoculated with Helminthosporium maydis or Helminthosporium carbonum. Physiology Plant Pathology, v.25, p.111-23, 1984.

FILIPPI, M.C.; SILVA, G.B.; PRABHU A.S. Indução de resistência à brusone em folhas de arroz por isolado avirulento de Magnaporthe oryzae. Fitopatologia Brasileira, v.32, p.387-392, 2007.

FUJITA, Y.; SONDA, R.; YAEGESHI, H. Leaf blast suppression by pre-inoculation of some incompatible lesion-type isolates of Pyricularia oryzae. Annals of the Phytopathological Society of Japan, v.56, p.23-275, 1990.

INTERNATIONAL RICE RESEARCH INSTITUTE. Standard evaluation system for rice. International rice testing program. 3nd ed. Los Baños, 1988. 54p.

KEESEY, J. Biochemica information. Indianapolis: Boehringer Manhein Biochemicals, 1987. 58p.

KLOEPPER J.W.; TUZUN, S.; KUC, J.A. Proposed definitions related to induced disease resistance. Biocontrol Science and Technology, v.2, p.349-51, 1992.

MANANDHAR, H.J.; LYNGS-JORGENSEN, H.J.; MATHUR, S.B.; SMEDEGAARG-PETERSEN, V. Resistance to rice blast induced by ferric chloride, dipotassium hydrogen phosphate and salicylic acid. Crop Protection, v.17, p.323-329, 1998.

NISHIZAWA, Y.; HIBI, T. Rice chitinase gene: cDNA cloning and stress-induced expression. Plant Science, v.76, p.211-218, 1991.

NOTTEGHEM, J.L. Cooperative experiment on horizontal resistance to rice blast. In: BLAST and upland rice: report and recommendations from the meeting for international collaboration in upland rice improvement. Los Baños: International Rice Research Institute, 1981. p.43-51.

OHATA, K.; KOZAKA, T. Interaction between two races of Pyricularia oryzae in lesion formation in rice plants and accumulation of fluorescent compounds associated with infection. Bulletin of the National institute of Agricultural Sciences, v.21, p.111-135, 1967.

OUYANG, G.C.; YING, C.Y.; ZHU, M,H.; XUE, Y.L. Induction of disease resistance by spores and toxins of Pyricularia oryzae in rice and its relation to the phenylpropane pathway. Plant Physiology Communications, v.4, p.40, 1987

PAN, S.Q; YE, X.S.; KUC, J. Association of a -1,3-glucanase activity and isoform pattern with systemic resistance to blue mold in tobacco induced by stem injection with Peronospora tabacina or leaf inoculation with tobacco mosaic virus. Physiology Molecular Plant Pathology, v.39, p.25-39, 1991.

PARK, S.K.; KIM, K.C. Effects of mixing and reciprocal inoculation with compatible and incompatible races of Pyricularia oryzae on the enlargement of disease lesions of rice blast. Korean Journal of Plant Protection, v.22, p.300-306, 1983.

PIETERSE, C.M.J.; VAN LOON, L.C. NPR1: the spider in the web of induced resistance signaling Pathways. Current Opinion in Plant Biology, v.7, p.456-464, 2004.

PRABHU, A.S.; FILIPPI, M.C.; SILVA, G.B.; LOBO, V.L.S; MORAES, O.P. An unprecedented outbreak of rice blast on a newly released cultivar BRS Colosso in Brazil. In.: WANG, G.L.; VALENT, B. (Eds.). Advances in genetics, genomics and control of rice blast disease. New York: Springer, 2009. p.257-266.

REIMMANN, C.R.; RINGLI, C.; DUDLER, R. Complementary DNA cloning and sequence analysis of a pathogen-induced peroxidase from rice. Plant Physiology, v.100, p.1611-1612, 1992.

SHANER, G.; FINNEY, R.E. The effect of nitrogen fertilization on the expression of slow mildewing resistance in knox wheat. Phytopathology, v.67, p.1051-1056, 1977.

SILVERMAN, P.; SESKAR, M.; KANTER, D.; SCHWEIZER, P.; METRAUX, J.P.; RASKIN, I. Salicylic acid in rice. Plant Physiology, v.108, p.633-639, 1995.

SIMMONS, C.R.; LITTS, J.C.; HUANG, N.; RODRIGUEZ, R.L. Structure of rice B-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors. Plant Molecular Biology, v.18, p.33-45, 1992.

SMITH, J.A.; METRAUX, J.P. Pseudomonas syringae cv. syringae induces systemic resistance to Pyricularia oryzae in rice. Physiological and Molecular Plant Pathology, v.39, p.451-461, 1991.

TAIZ, L.; ZEIGER, E. Plant Physiology. 4th ed. Sunderland: Sinauer, 2006. 700p.

WANG, Z.X.; YANO, M.; YAMANOUCHI, U.; IWAMOTO, M.; MONNA, L.; HAYASAKA, H.; KATAYOSE, Y.; SASAKI, T. The Pib gene for rice blast resistance belongs to the nucleotide binding, leucinerich repeat class of plant disease resistance genes. Plant Journal, v.19, p.55–64, 1999.

YAO, H.; TIAN, S. Effects of pre- and post-harvest application of salycic acid or methyl jasmonate on inducing disease resistance of sweet cherry in fruit storage. Postharvest Biology and Technology, v.35, p.253-262, 2005.

ZIMMERLI, L.; JAKAB, G.; METRAUX, J.P.; MAUCH-MANI, B. Potentiation of pathogen-specif defense mechanisms in Arabidopsis by β-aminobutiric acid. Proceedings of the National Academy of Sciences of the United States of America, v.161, p.557-568, 2004.

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Publicado

2014-11-24

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Artigos Científicos